1/7) How does global #mitochondria protein import look like in unperturbed or stressed cells? Check out our new paper (open access), which presents a #proteomics method and shows the contribution of #translation and translocation to protein import. cell.com/molecular-cell/full…

6:30 PM · Nov 29, 2021

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2/7) Based on our recently developed method for protein translation proteomics (doi.org/10.1016/j.molcel.201…), we now added specific booster/carrier signals that allow monitoring new imported (i.e. newly translated) proteins inside mitochondria.
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3/7) Using this import proteomics assay, we quantified uptake rates for over 700 mitochondrial proteins, observing a dynamic range over six order of magnitude. The paper shows a number of bioinformatics analyses to define features guiding import.
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4/7) We then moved to characterize the effects of two different sources of mitochondrial import stress (MitoBloCK6 and CCCP). Both compounds had distinct effects on mitochondrial sub-compartments. Importantly, a number of proteins continued to be imported.
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5/7) Strikingly, of the proteins showing decreased import during depolarization, one subset was driven by decreases in translocation, while another set of proteins showed decreased translation, which caused decreased uptake. This is driven by the integrated stress response.
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6/7) To be able to better analyse pulsed SILAC-TMT experiments, we created a novel tool called DynaTMT that can be used for the post-processing data extraction and normalisation. It is available as an interactive tool (PC, Mac, Linux) and python package. github.com/klannk/DynaTMT
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7/7) Great work by a team of co-first authors @JasminASchaefer, Süleyman @science_mbg and @jonas_michaelis, together with support from @BobbyKlaus3. Made possible with the generous support from @ERC_Research, @dfg_public, @CPI_ExStra
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Replying to @MuenchLab
Absolutely beautiful work - congratulations! 🎉
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Thanks, highly appreciated!
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Replying to @MuenchLab
Congrats @MuenchLab!!
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